Anti-Fibrillarin Antibody (A13296)

Name: Anti-Fibrillarin Antibody
Brand: Antibodies.com
SKU: A13296
Price: 280.0 GBP
Availability: InStock

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This product is discontinued

Anti-Fibrillarin Antibody (A13296) has been discontinued and is no longer available.

View all Fibrillarin Antibodies.

Specifications

Name

Anti-Fibrillarin Antibody

Description

Rabbit polyclonal antibody to Fibrillarin.

Applications

WB, ICC/IF, IP, ELISA

Positive Control

HeLa, A-375, U2OS, COS1, COS7, PC-12, Mouse brain, Mouse testis.

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200, IP: 0.5µg-4µg antibody for 200µg-400µg extracts of whole cells, ELISA: 1 µg/ml (starting concentration)

Reactivity

Human, Mouse, Rat, Monkey

Immunogen

A recombinant fusion protein corresponding to amino acids 100-321 of human Fibrillarin.

Sequence

RGKEDALVTKNLVPGESVYGEKRVSISEGDDKIEYRAWNPFRSKLAAAILGGVDQIHIKPGAKVLYLGAASGTTVSHVSDIVGPDGLVYAVEFSHRSGRDLINLAKKRTNIIPVIEDARHPHKYRMLIAMVDVIFADVAQPDQTRIVALNAHTFLRNGGHFVISIKANCIDSTASAEAVFASEVKKMQQENMKPQEQLTLEPYERDHAVVVGVYRPPPKVKN

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

36 kDa

Predicted MW

34 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

34 kDa nucleolar scleroderma antigen, FBL, FIB1, FLRN, Histone-glutamine methyltransferase, rRNA 2'-O-methyltransferase fibrillarin, U6 snRNA 2'-O-methyltransferase fibrillarin

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-Fibrillarin Antibody (A13296)

Fibrillarin expression in various lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-Fibrillarin Antibody (A13296) at a dilution of 1:1000 and detected with chemiluminescence.

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Immunofluorescence - Anti-Fibrillarin Antibody (A13296)

Fibrillarin expression in Hela cells analyzed by immunofluorescence. Staining was performed with Anti-Fibrillarin Antibody (A13296) at a dilution of 1:100. Nuclei were stained with DAPI (blue).

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Immunofluorescence - Anti-Fibrillarin Antibody (A13296)

Fibrillarin expression in U2OS cells analyzed by immunofluorescence. Staining was performed with Anti-Fibrillarin Antibody (A13296) at a dilution of 1:200. Nuclei were stained with DAPI (blue).

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Immunoprecipitation - Anti-Fibrillarin Antibody (A13296)

Fibrillarin expression in 200 µg extracts of HeLa cells analyzed by western blot following immunoprecipitation, both using Anti-Fibrillarin Antibody (A13296) at 1 µg and a dilution of 1:1000, respectively.