Rabbit polyclonal antibody to HER2 (Phospho-Tyr877)
Specificity
The antibody detects endogenous level of HER2 only when phosphorylated at tyrosine 877.
Applications
WB, IHC, IF
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of tyrosine 877 (T-E-Y(p)-H-A) derived from Human HER2.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using HER2 (Phospho-Tyr877) Antibody #11075 (left) or the same antibody preincubated with blocking peptide (right).
![Flow Cytometry - Anti-HER2 Antibody [ERB2/776] (A248455) - Antibodies.com](https://cdn.antibodies.com/image/catalog/248/A248456_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-HER2 Antibody [ERBB2/4376R] - BSA and Azide free (A278563) - Antibodies.com](https://cdn.antibodies.com/image/catalog/278/A278563_1.jpg?profile=product_alternative)
![Immunofluorescence - Anti-HER2 Antibody [HRB2/451] - BSA and Azide free (A251630) - Antibodies.com](https://cdn.antibodies.com/image/catalog/251/A251630_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-HER2 Antibody [ERBB2/4439] - BSA and Azide free (A251641) - Antibodies.com](https://cdn.antibodies.com/image/catalog/251/A251641_1.jpg?profile=product_alternative)
![Immunofluorescence - Anti-HER2 Antibody [HRB2/451] (A248448) - Antibodies.com](https://cdn.antibodies.com/image/catalog/248/A248448_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-HER2 Antibody [ERBB2/2452] - BSA and Azide free (A251625) - Antibodies.com](https://cdn.antibodies.com/image/catalog/251/A251625_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-HER2 Antibody [ERBB2/4439] (A248459) - Antibodies.com](https://cdn.antibodies.com/image/catalog/248/A248459_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-HER2 Antibody [ERBB2/2452] (A248443) - Antibodies.com](https://cdn.antibodies.com/image/catalog/248/A248443_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-HER2 Antibody [ZR5] (A248458) - Antibodies.com](https://cdn.antibodies.com/image/catalog/248/A248458_1.jpg?profile=product_alternative)