Anti-HLA DR + HLA DP Antibody [HL-38] (A85931)

Immunohistochemistry staining of human small intestine (paraffin-embedded sections) using Anti-HLA DR + HLA DP Antibody [HL-38] (A85931).

Anti-HLA DR + HLA DP Antibody [HL-38] (A85931) works in WB application under reducing and non-reducing conditions. Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Raji and Jurkat cell lines, mixed and heated (100°C, 5 min) with reducing and non-reducing SDS-loading buffer. Samples were resolved using 10% Tris-glycine SDS gel electrophoresis. Nitrocellulose membrane blot was probed with mouse IgG2a monoclonal antibody HL-38 (1 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Multiplex fluorescent Western blot detection was performed. HLA-DR/DP molecules were detected at ~25 kDa in Raji cell line.

Flow cytometry surface staining of human peripheral blood labeling HLA DR + HLA DP with Anti-HLA DR + HLA DP Antibody [HL-38] (A85931).