Mouse monoclonal [HL-40] antibody to HLA DR + HLA DP.
Specificity
This antibody reacts with common epitope on beta-chain of human HLA-DR and HLA-DP. DR and DP are the isotypes of human MHC Class II molecules expressed on antigen-presenting cells (APC; dendritic cells, B lymphocytes, monocytes, macrophages).
Applications
Flow Cytometry, IP, IHC-P
Dilutions
Flow Cytometry: 1-5 µg/ml.
Reactivity
Human, Porcine
Immunogen
Raji Burkitt's lymphoma cell line.
Host
Mouse
Clonality
Monoclonal
Clone ID
HL-40
Isotype
IgG2a
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Separation of human HLA-DR/DP positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry surface staining of human peripheral whole blood, labeling HLA DR + HLA DP with Anti-HLA DR + HLA DP Antibody [HL-40] (A85917), (concentration in sample 1 µg/ml) GAM APC.