Anti-Lamin A (phospho Ser22) Antibody (A87589)

Western blot analysis of extracts of various cell lines, using Anti-Lamin A (phospho Ser22) Antibody (A87589) at 1:2,000 dilution or Lamin A antibody (A0249). HeLa cells were treated by Hydroxyurea (4 mM) at 37°C for 20 hours or treated by Paclitaxel (100 nM/mL) at 37°C for 20 hours. C6 cells were treated by Hydroxyurea (4 mM) at 37°C for 20 hours or treated by Paclitaxel (100 nM) at 37°C for 20 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 1s.

Immunoprecipitation analysis of 200µg extracts of HeLa cells using 3µg of Anti-Lamin A (phospho Ser22) Antibody (A87589). This Western blot was performed on the immunoprecipitate using Anti-Lamin A (phospho Ser22) Antibody (A87589) at a dilution of 1:1000. HeLa cells were treated by Paclitaxel (100 nM/mL) at 37°C for 20 hours.