Anti-MAP2 Antibody [4H5] (A85297)

Western blot analysis of tissue and cell lysates using Anti-MAP2 Antibody [4H5] (A85297), at a dilution of 1:10,000, in green. The lanes contain samples of: [Lane 1] Protein standards, in red, [Lane 2] rat brain, [Lane 3] mouse brain, and [Lane 4] embryonic rat cortical neuron-glial cell lysate. A band at about 280 kDa corresponds to the MAP2A and MAP2B proteins.

Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with Anti-Galectin 3 Antibody (A104321), at a dilution of 1:2,000, in green, and co-stained with Anti-MAP2 Antibody [4H5] (A85297), at a dilution of 1:2,000, in red. The blue is Hoechst staining of nuclear DNA. Anti-Galectin 3 Antibody (A104321) produces strong staining of certain glial cells, while Anti-MAP2 Antibody [4H5] (A85297) labels dendrites and perikarya of mature neurons.

Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with Anti-Galectin 3 Antibody (A104321) at a dilution of 1:2,000 (green) and costained with Anti-MAP2 Antibody [4H5] (A85297) at a dilution of 1:2,000 (red). Nuclei were stained with Hoechst (blue). Anti-Galectin 3 Antibody (A104321) antibody produces strong staining of certain glial cells, while Anti-MAP2 Antibody antibody labels dendrites and perikarya of mature neurons.

Immunohistochemistry analysis of a 4% PFA fixed paraffin embedded rat hippocampus section with Anti-MAP2 Antibody [4H5] (A85297) at a dilution of 1:1,000 detected in DAB (brown) following the ImmPress method with Anti-MAP2 Antibody [4H5] (A85297) strongly labels neurons, neuronal projections, and synapses. Note: this antibody performs well in testing with 4% PFA fixed tissues but does not stain NBF fixed human material effectively. We recommend clone Anti-MAP2 Antibody [5H11] (A85296) for human tissue immunohistochemistry.

SDS-PAGE of Anti-MAP2 Antibody [4H5] (A85297). The lanes contain: [Lane S] protein standard, [Next 5 lanes] 2µg of purified recombinant forms of various human MAP2 proteins. Note: D is recombinant full length MAP2D (559 amino acids) and C is recombinant full length MAP2C (471 amino acids), which is missing one of the microtubule binding domains of MAP2D and a more N-terminal 57 amino acid N-terminal sequence. Lanes P1, P2 and P3 show each recombinant segments of the human MAP2 projection domain, found in MAP2A and B but absent from MAP2C and D. P1: amino acids 233-684, P2: 712-1136, and P3: 1137-1588. All of the constructs run more slowly on SDS-PAGE than expected from their molecular size, probably due to their high content of charged amino acids. These constructs were used to make a series of mouse monoclonal antibodies. The lane marked BSA contains 2µg of bovine serum albumin, another gel standard.