Rabbit polyclonal antibody to MARCKS (Phospho-Ser158)
Specificity
The antibody detects endogenous level of MARCKS only when phosphorylated at serine 158.
Applications
WB, ICC/IF
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of serine 158 (R-F-S(p)-F-K) derived from Human MARCKS.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
80K-L protein, MACS, Myristoylated alanine-rich C-kinase substrate, PKCSL, PRKCSL, Protein kinase C substrate, 80 kDa protein, light chain
Western blot analysis of extracts from 3T3 cells using MARCKS (Phospho-Ser158) Antibody #11293 (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).
![Immunofluorescence - Anti-MARCKS Antibody [5F9] (A270556) - Antibodies.com](https://cdn.antibodies.com/image/catalog/270/A270556_1.jpg?profile=product_alternative)
