Anti-MARCKS Antibody (A85457)

Immunofluorescent analysis of HeLa cells stained with Anti-MARCKS Antibody, at a dilution of 1:1,000, in red, and Anti-Beta Tubulin Antibody (A85428 | 1:10,000, in green. The blue is DAPI staining of nuclear DNA. Anti-MARCKS Antibody recognizes protein expressed in the plasma membrane and cytoplasm, while the Anti-Beta Tubulin Antibody stains microtubules.

View of mixed neuron/glial cultures stained with Anti-MARCKS Antibody (red) and Anti-MAP2 Antibody (A85363 | green). Note that the Anti-MARCKS Antibody stains vesicular structures both in the glial cells and in the dendrites of the neurons, which are strongly stained with the Anti-MAP2 Antibody.

Western blot analysis of cell line lysates probed simultaneously with Anti-MARCKS Antibody, at a dilution of 1:1,000, in green, and Anti-GAPDH Antibody (A85382 | 1:5,000, in red,: [Lane 1] protein standard (red), [Lane 2] HEK293, [Lane 3] HeLa, [Lane 4] SH-SY5Y, [Lane 5] COS1, [Lane 6] NIH-3T3, [Lane 7] C6 cells. The strong band at 80kDa corresponds to MARCKS protein, detected only in the proteins of human origin. Slight reactivity is observed in the monkey cells [Lane 5], but no reactivity is seen on rodent cells. Anti-GAPDH Antibody was used as a loading control - revealing a single band at ~37kDa in all preparations.

Immunohistochemistry analysis of a formalin fixed paraffin embedded human hippocampus section with Anti-MARCKS Antibody (A85457) at a dilution of 1:2,000. Anti-MARCKS Antibody (A85457) labels the nuclei, membrane and cytoplasm of glial cells and the synapses of neuronal cells. Note: this antibody performs well in the staining of NBF fixed human tissues but does not cross react with rodent.