Western Blot - Anti-MARCKS (phospho Ser162) Antibody (A16630)
Western blot analysis of various lysates, using Anti-MARCKS (phospho Ser162) Antibody (A16630) at 1:400 dilution. 293T cells were treated by PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.