Western blot analysis of various lysates, using Anti-MIF Antibody (A308197) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Western blot analysis of various lysates, using Anti-MIF Antibody (A308197) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Western blot analysis of extracts from wild type(WT) and MIF Rabbit polyclonal antibody knockout (KO) 293T cells, using Anti-MIF Antibody (A308197) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
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Alternative products to Anti-MIF Antibody (A308197)
Recombinant human monoclonal antibody to MIF for use as a research grade Imalumab biosimilar for ELISA, Flow Cytometry, Functional Studies and in vivo Research.
Recombinant human monoclonal antibody to MIF for use as a research grade Imalumab biosimilar for ELISA, Flow Cytometry, Functional Studies and in vivo Research.
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