This antibody recognises homeobox protein NANOG, otherwise known as homeobox transcription factor NANOG.NANOG plays a role in the proliferation and self-renewal of embryonic stem (ES) cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Overexpression of NANOG has been shown to promote cells to enter into S phase and proliferation.
A peptide derived from within the N-terminus of mouse NANOG.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.035% Sodium Azide and 30% Glycerol.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Rabbit anti Mouse NANOG antibody recognizes homeobox protein NANOG, otherwise known as homeobox transcription factor NANOG.NANOG plays a role in the proliferation and self-renewal of embryonic stem (ES) cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages (Barral et al. 2019). Overexpression of NANOG has been shown to promote cells to enter into S phase and proliferation (Sainz de Aja et al. 2019).
Synonyms
hNanog, Homeobox protein NANOG, Homeobox transcription factor Nanog
Immunofluorescence staining of mouse embryonic stem cells (mESCs) grown on mouse embryonic fibroblast feeder cells (MEFs) probed with Anti-Nanog Antibody (A320157) at a 1/200 dilution.
Western Blot analysis of P19 nuclear extract (lane 1), P19 nuclear extract treated with retinoic acid (lane 2) and mouse ES nuclear extract (lane 3) probed with Anti-Nanog Antibody (A320157) at a 1/500 dilution.
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