Western blot analysis of extracts of Neuro-2a cells, using Anti-Nestin Antibody (A81194) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-Nestin Antibody (A81194) at dilution of 100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Nestin Antibody (A81194) at dilution of 100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of Neuro-2a cells using Anti-Nestin Antibody (A81194) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
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