Rabbit polyclonal antibody to NPM (Phospho-Thr199)
Specificity
The antibody detects endogenous level of NPM only when phosphorylated at Threonine 199.
Applications
WB
Reactivity
Human, Mouse
Immunogen
Peptide sequence around phosphorylation site of Threonine 199 (R-D-T(p)-P-A) derived from Human NPM.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C / 1 year
Synonyms
Nucleolar phosphoprotein B23, Nucleolar protein NO38, Nucleophosmin, Numatrin
Western blot analysis of extracts from Mouse heart tissue using NPM (Phospho-Thr199) Antibody #12031.The lane on the left is treated with the antigen-specific peptide.
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