Recombinant Anti-Nucleophosmin Antibody [rNPM1/8056] (A315416)

Anti-Nucleophosmin Antibody [rNPM1/8056]

Recombinant mouse monoclonal [rNPM1/8056] antibody to Nucleophosmin.

Recognizes a 33kDa glycoprotein, identified as Nucleophosmin (NPM). It is predominantly localized in the nucleus of cells in most tissues. NPM is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis. This phosphoprotein moves between the nucleus and the cytoplasm. It is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location. Mutations in this gene are associated with acute myeloid leukemia.The antibody may be a useful aid for classification of acute myeloid leukemia.

K562, MCF-7, HeLa or A431 cells. Human skin or colon tissue (IHC).

IHC: 1-2 µg/ml

Human

Recombinant fragment of human NPM1 protein. The exact sequence is proprietary.

Mouse

Monoclonal

rNPM1/8056

kappa

Protein A/G chromatography.

200 µg/ml

33 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

NPM, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Numatrin