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Anti-PCNA Antibody (A34153)

Immunocytochemistry - PCNA Monoclonal Antibody from Signalway Antibody (27210) - Antibodies.com Western blot - PCNA Monoclonal Antibody from Signalway Antibody (27210) - Antibodies.com Immunoprecipitation - PCNA Monoclonal Antibody from Signalway Antibody (27210) - Antibodies.com Immunohistochemistry - PCNA Monoclonal Antibody from Signalway Antibody (27210) - Antibodies.com
This product is discontinued

Anti-PCNA Antibody (A34153) has been discontinued and is no longer available.

View all PCNA Antibodies.

  • Specifications

Name
Anti-PCNA Antibody
Description
Mouse monoclonal antibody to PCNA
Specificity
This antibody detects endogenous levels of PCNA and does not cross-react with related proteins.
Applications
WB, IP, ICC/IF, IHC
Dilutions
Western blotting: 1:1000
Reactivity
Human, Mouse, Monkey, Rat
Immunogen
Purified recombinant human PCNA protein fragments expressed in E.coli.
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Conjugate

Unconjugated

Formulation
Supplied as ascites fluid.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Cyclin, Proliferating cell nuclear antigen
Isotype Controls
Suitable Secondaries
See all Anti-Mouse IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (4)

Immunocytochemistry - PCNA Monoclonal Antibody from Signalway Antibody (27210) - Antibodies.comEnlarge Image

Immunocytochemistry - Anti-PCNA Antibody (A34153)

Immunocytochemistry staining of HeLa cells using anti-PCNA antibody (dilution 1: 100).Fixed in 100% methanol for 2hr at -20℃.
Western blot - PCNA Monoclonal Antibody from Signalway Antibody (27210) - Antibodies.comEnlarge Image

Anti-PCNA Antibody (A34153)

Western blot detection of PCNA antibody in Hela,3T3,COS7,C6 and CHO-K1 cell lysates using PCNA antibody (1: 1000 diluted).Predicted band size: 36KDa.Observed band size: 36KDa.
Immunoprecipitation - PCNA Monoclonal Antibody from Signalway Antibody (27210) - Antibodies.comEnlarge Image

Immunoprecipitation - Anti-PCNA Antibody (A34153)

Immunoprecipitation analysis of Hela cell lysates using PCNA antibody.
Immunohistochemistry - PCNA Monoclonal Antibody from Signalway Antibody (27210) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-PCNA Antibody (A34153)

Immunohistochemical analysis of paraffin-embedded human colorectal carcinoma with PCNA Mouse mAb (2E1-G10-H10,1: 400 diluted),showing nuclear localization. A high pressure mediated antigen retrieval step was performed in citrate buffer (pH6.0).

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Proteins predicted to interact with PCNA

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score