Anti-PCNA Antibody [PC10] (A281549)

Anti-PCNA Antibody [PC10]

Mouse monoclonal [PC10] antibody to PCNA.

This antibody recognises the proliferating cell nuclear antigen, also known as PCNA or cyclin. PCNA is a 261 amino acid ~28 kDa nuclear protein vital for cellular DNA synthesis at the replication fork through its interaction with FEN1. PCNA is the auxilliary protein for DNA polymerase d. PCNA is highly conserved between mammalian species and other vertebrates. Mouse anti PCNA antibody, clone PC10 has been used for the detection of PCNA in a number of species including human, rat, mouse , chicken (Franz-Odendaal 2008) and abalone.

WB, IHC-P, IHC-Fr, Flow Cytometry, IP

FC 1: 1:100, IHC-P: 1:50 - 1:100, WB: 1:1,000, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl

Rat, Vertebrates, Invertebrates, Ferret, Chicken, Rabbit, Xenopus, Mouse, Horse, Sheep, Dog, Cat, Cynomolgus monkey, Rhesus Monkey, Hamster, Atlantic Salmon, Human, Bearded Dragon, Corn Snake, Nile Crocodile

Rat PCNA made in the protein A expression vector pR1T2T.

Mouse

Monoclonal

PC10

IgG2a

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti PCNA antibody, clone PC10 recognizes the proliferating cell nuclear antigen, also known as PCNA or cyclin. PCNA is a 261 amino acid ~28 kDa nuclear protein vital for cellular DNA synthesis at the replication fork (Li et al. 1995) through its interaction with FEN1 (Wu et al. 1996). PCNA is the auxilliary protein for DNA polymerase d (Bravo et al. 1987).PCNA is highly conserved between mammalian species and other vertebrates. Mouse anti PCNA antibody, clone PC10 has been used for the detection of PCNA in a number of species including human, rat, mouse (Park et al. 2008), chicken (Franz-Odendaal 2008) and abalone (Harris et al. 2006).

Cyclin, Proliferating cell nuclear antigen