Rabbit polyclonal antibody to SirT1 (Phospho-Ser47)
Specificity
The antibody detects endogenous level of SirT1 only when phosphorylated at serine 47.
Applications
WB
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of serine 47(E-R-S(p)-P-G) derived from Human SirT1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
hSIR2, hSIRT1, NAD-dependent protein deacetylase sirtuin-1, NAD-dependent protein deacylase sirtuin-1, Regulatory protein SIR2 homolog 1, SIR2-like protein 1, SIR2L1
Western blot analysis of extracts from JK tissue using SirT1 (Phospho-Ser47) antibody #11926.The lane on the right is treated with the antigen-specific peptide.
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