Rabbit polyclonal antibody to SSTR2 (phospho Ser341 + Ser343).
Specificity
This antibody specifically targets the phosphorylated form of human SSTR2 at residues Ser341 and Ser343. It detects agonist-induced phosphorylation in Western blot analysis and supports detection in mouse tissues in vivo.
Applications
WB
Dilutions
WB: 1:1,000
Reactivity
Human, Mouse
Immunogen
Synthetic phosphopeptide derived from human SSTR2 around the phosphorylation site of Ser341 + Ser343. Immunogen range is 10-12 amino acids.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Dulbecco's PBS, pH 7.4, with 150 mM NaCl and 0.005% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Somatostatin receptor type 2, SRIF-1, SS-2-R, SS2-R, SS2R, SST2
Analysis of agonist-induced phosphorylation of Somatostatin Receptor 2 (SSTR2) at Serine341/Serine343. HEK293 cells stably expressing SSTR2 were untreated or treated with 1 µM SRIF or 1 µM Octreotide for 30 min. Lysates were immunoblotted with anti-SSTR2 (phospho Ser341 + Ser343) antibody (A334540) at 1:1000 (upper panel). The blot was stripped and reprobed with phosphorylation-independent anti-SSTR2 antibody (A334543) at 1:1000 to confirm equal loading (lower panel).
Dose-dependent phosphorylation analysis of Somatostatin Receptor 2 (SSTR2) using phosphosite-specific antibodies. HEK293 cells stably expressing SSTR2 were untreated or exposed to 1 nM to 10 µM SRIF for 30 min. Lysates were immunoblotted with anti-SSTR2 (phospho Ser341 + Ser343) (A334540), anti-SSTR2 (phospho Thr353 + Thr354), or anti-SSTR2 (phospho Thr356 + Thr359) (A334541) antibodies at 1:1000 (upper panels). The blot was stripped and reprobed with phosphorylation-independent anti-SSTR2 antibody (A334543) at 1:1000 to confirm equal loading (lower panel).
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