This antibody binds specifically to the C-terminal region of human SST3, detecting the non-phosphorylated form. It is optimized for Western blot analysis, facilitates immunoprecipitation from tissue lysates, and supports immunohistochemistry in cultured cells and tissue sections.
Applications
WB, ICC, IHC
Dilutions
WB: 1,000, IHC: 1:100, ICC: 1:200
Reactivity
Human
Immunogen
Synthetic peptide corresponding to human SST3 (amino acids. 398-418). Immunogen range is 22-22 amino acids.
Sequence
QLLPQEASTGEKSSTMRISYL
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Dulbecco's PBS, pH 7.4, with 150 mM NaCl and 0.005% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Somatostatin receptor type 3, SS-3-R, SS3-R, SS3R, SSR-28, SST3
Western blot validation of Somatostatin Receptor 3 (SST3) in transfected HEK293 cells. Lysates from native HEK293 cells (MOCK) or cells stably expressing SST3 were probed with phosphorylation-independent anti-SST3 antibody (A334545) at 1:1000.
Immunocytochemical detection of Somatostatin Receptor 3 (SST3) in HEK293 cells. HEK293 cells stably expressing SST3 were untreated or treated with 1 µM SRIF for 30 min and stained with anti-SST3 antibody (A334545) at 1:200. SST3 receptors were confined to the plasma membrane in untreated cells (0 min) and observed in perinuclear vesicular clusters after 30 min of SRIF exposure.
Immunohistochemical localization of Somatostatin Receptor 3 (SST3) in human growth-hormone-producing pituitary adenoma. Tumor sections were dewaxed, microwaved in citric acid, and stained with anti-SST3 antibody (A334545) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed with 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. SST3 receptors were uniformly detected at the plasma membrane of nearly all tumor cells.
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