This antibody reacts with human vimentin, a 57 kDa intermediate filament protein expressed on a wide variety of mesenchymal and mesodermal cell types.
Applications
IHC-P, Flow Cytometry, WB, ICC, ELISA
Dilutions
ELISA: The antibody VI-RE/1 recognizes different epitope on human vimentin than the antibody VI-01 (IgM), Flow Cytometry: 1-4 µg/ml; Intracellular staining, WB: 1-2 µg/ml, IHC: 5-10 µg/ml.
Reactivity
Human
Cross Reactivity
This antibody does not cross react with Mouse or Pig.
Immunogen
Bacterially expressed full length human Vimentin protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
VI-RE/1
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
57 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Immunohistochemistry staining (paraffin sections) of vimentin in human liver using Anti-Vimentin Antibody [VI-RE/1] (A86554), (diluted 1:400), detected with GAM IgG-Alexa Fluor®488 (diluted 1:200; green), cell nuclei stained with PI (1µg/ml; orange).
Western blotting analysis of human vimentin using Anti-Vimentin Antibody [VI-RE/1] (A86554) on lysates of MOLT-4 cell line (low expression), U87 cell line (positive) and Raji cell line (negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-vimentin monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for vimentin at approximately 55 kDa.
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