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Anti-Vimentin (phospho Ser56) Antibody (A94802)

Western Blot - Anti-Vimentin (phospho Ser56) Antibody (A1243) - Antibodies.com Immunohistochemistry - Anti-Vimentin (phospho Ser56) Antibody (A1243) - Antibodies.com Western Blot - Anti-Vimentin (phospho Ser56) Antibody (A1243) - Antibodies.com
This product is discontinued

Anti-Vimentin (phospho Ser56) Antibody (A94802) has been discontinued and is no longer available.

View all Vimentin Antibodies.

  • Specifications

Name
Anti-Vimentin (phospho Ser56) Antibody
Description
Rabbit polyclonal antibody to Vimentin (phospho Ser56).
Specificity
This antibody detects endogenous levels of Vimentin only when phosphorylated at Ser56.
Applications
WB, IHC, ELISA
Dilutions
WB: 1:500~1:1000, ELISA: 1:10000
Reactivity
Human
Immunogen
Synthetic peptide derived from human Vimentin around the phosphorylation site of Ser56 (amino acids 31-80).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
53kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
VIM
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-Vimentin (phospho Ser56) Antibody (A1243) - Antibodies.comEnlarge Image

Western Blot - Anti-Vimentin (phospho Ser56) Antibody (A94802)

Western blot analysis of lysates from A549 cells treated with Nocodazole 1µg/ml 16h using Anti-Vimentin (phospho Ser56) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Immunohistochemistry - Anti-Vimentin (phospho Ser56) Antibody (A1243) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-Vimentin (phospho Ser56) Antibody (A94802)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Anti-Vimentin (phospho Ser56) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Western Blot - Anti-Vimentin (phospho Ser56) Antibody (A1243) - Antibodies.comEnlarge Image

Western Blot - Anti-Vimentin (phospho Ser56) Antibody (A94802)

Western blot analysis of A549 cells using Anti-Vimentin (phospho Ser56) Antibody.

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Proteins predicted to interact with Vimentin

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
95.7% Relevancy Score
93.4% Relevancy Score
93.2% Relevancy Score
92.7% Relevancy Score
92.3% Relevancy Score
91.6% Relevancy Score
90.8% Relevancy Score
90% Relevancy Score
89.9% Relevancy Score