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Anti-CD34 Antibody (A87833)

Western Blot - Anti-CD34 Antibody (A87833) - Antibodies.com Immunohistochemistry - Anti-CD34 Antibody (A87833) - Antibodies.com Immunohistochemistry - Anti-CD34 Antibody (A87833) - Antibodies.com Immunohistochemistry - Anti-CD34 Antibody (A87833) - Antibodies.com Immunofluorescence - Anti-CD34 Antibody (A87833) - Antibodies.com Immunofluorescence - Anti-CD34 Antibody (A87833) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to CD34 for WB, IHC and ICC/IF.

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Anti-CD34 Antibody
Goat polyclonal antibody to CD34 for ELISA and IF.
Name
Anti-CD34 Antibody
Description
Rabbit polyclonal antibody to CD34.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human CD34 (NP_001020280.1).
Sequence
SNVSTNVSYQETTTPSTLGSTSLHPVSQHGNEATTNITETTVKFTSTSVITSVYGNTNSSVQSQTSVISTVFTTPANVSTPETTLKPSLSPGNVSDLSTTS
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
105 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
CD34 antigen, CD34 molecule, CD34_HUMAN, Cluster designation 34, Hematopoietic progenitor cell antigen CD34, HPCA1, Mucosialin, OTTHUMP00000034733, OTTHUMP00000034734
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-CD34 Antibody (A87833) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-CD34 Antibody (A87833)
Western blot analysis of extracts of various cell lines, using Anti-CD34 Antibody (A87833) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
Immunohistochemistry - Anti-CD34 Antibody (A87833) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-CD34 Antibody (A87833)
Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-CD34 Antibody (A87833) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-CD34 Antibody (A87833) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-CD34 Antibody (A87833)
Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-CD34 Antibody (A87833) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-CD34 Antibody (A87833) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-CD34 Antibody (A87833)
Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-CD34 Antibody (A87833) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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