Anti-CD34 Antibody [QBEnd/10] (A250677)

Anti-CD34 Antibody [QBEnd/10]

Mouse monoclonal [QBEnd/10] antibody to CD34.

This antibody recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. On the basis of differential sensitivity to degradation by specific enzymes, epitopes of monoclonal antibodies to CD34 are classified intothree main categories, class I, class II and class III. It is a class II antibody whose epitope is resistant to neuraminidase but sensitive to glycoprotease and chymopapain.CD34 expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, CD34 is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. It appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. Anti-CD34 labels 85% of angiosarcoma and Kaposis sarcoma, but with a lower specificity.

Flow Cytometry, IF, IHC-P

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, IHC-P: 0.25-0.5 µg/ml

Human, Cynomolgus Monkey, Rhesus Monkey

This antibody does not cross react with Rat, Sheep, Bovine, or Canine.

Detergent solubilized vesicular suspension prepared from human term placenta.

Mouse

Monoclonal

QBEnd/10

IgG1

Unconjugated

Protein A/G chromatography.

200 µg/ml

90-110 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-CD34 Antibody [QBEnd/10] - BSA and Azide free (A253857).

CD34 antigen, CD34 molecule, CD34_HUMAN, Cluster designation 34, Hematopoietic progenitor cell antigen CD34, HPCA1, Mucosialin, OTTHUMP00000034733, OTTHUMP00000034734