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Anti-CD45 Antibody

 
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Total Citations (32)
Mouse monoclonal (MEM-28) antibody to CD45 from Antibodies.com (A85889).

   Please Note: This is exactly the same antibody that is marketed as:


 
   •  Abcam | ab8216
   •  Novus Biologicals | NB500-319
Name: Anti-CD45 Antibody
Description: Mouse monoclonal (MEM-28) antibody to CD45.
Specificity: The antibody MEM-28 reacts with all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets.
Applications: FC, IP, WB, IHC-P, ICC
Reactivity: Human
Immunogen: Human thymocytes and T lymphocytes.
Host: Mouse
Clonality: Monoclonal
Clone: MEM-28
Isotype: IgG1
Conjugate: Unconjugated
Purification: Protein A chromatography.
Concentration: 1 mg/ml
Purity: > 95% (by SDS-PAGE)
Product Form: Liquid
Formulation: Supplied in Phosphate Buffered Saline, pH 7.4, with 15mM Sodium Azide.
Storage: Store at 2-8°C.
Introduction: CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
Synonyms: B220 Antibody
CD 45 Antibody
CD45 antigen Antibody
CD45R Antibody
CD45RA Antibody
CD45RB Antibody
CD45RO Antibody
EC 3.1.3.48 Antibody
Glycoprotein Antibody
GP180 Antibody
L CA Antibody
L-CA Antibody
LCA Antibody
Leukocyte common antigen Antibody
Leukocyte common antigen precursor Antibody
loc Antibody
LY 5 Antibody
Ly-5 Antibody
LY5 Antibody
Ly5, homolog of Antibody
Lymphocyte antigen 5 Antibody
Lyt-4 Antibody
Lyt4 Antibody
OTTHUMP00000033813 Antibody
OTTHUMP00000033816 Antibody
OTTHUMP00000033817 Antibody
OTTHUMP00000038574 Antibody
Protein tyrosine phosphatase receptor type C Antibody
Protein tyrosine phosphatase receptor type c polypeptide Antibody
protein tyrosine phosphatase, receptor type, C Antibody
Protein tyrosine phosphatase, receptor type, c polypeptide Antibody
Ptprc Antibody
PTPRC_HUMAN Antibody
Receptor type tyrosine protein phosphatase C Antibody
Receptor-type tyrosine-protein phosphatase C Antibody
RT7 Antibody
T200 Antibody
T200 glycoprotein Antibody
T200 leukocyte common antigen Antibody

   Please Note: These publications cite this antibody under different brand names & catalogue numbers.


Cites NB500-319

Ilangumaran S, Briol A, Hoessli DC. CD44 selectively associates with active Src family protein tyrosine kinases Lck and Fyn in glycosphingolipid-rich plasma membrane domains of human peripheral blood lymphocytes Blood

Cites ab8216

Horejsi­ V  et al. Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74). Folia Biol (Praha) 34:23-34 (1988).

Cites NB500-319

Horejsi V, Angelisova P, Bazil V et al. Monoclonal antibodies against human leucocyte antigens II Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74) Folia Biol (Praha) Jun 10 1905

Cites ab8216

Jackson MV & Krasnodembskaya AD Analysis of Mitochondrial Transfer in Direct Co-cultures of Human Monocyte-derived Macrophages (MDM) and Mesenchymal Stem Cells (MSC). Bio Protoc 7:N/A (2017).

Cites ab8216

Huo Z  et al. The relationship between allergic status and adenotonsillar regrowth: a retrospective research on children after adenotonsillectomy. Sci Rep 7:46615 (2017).

Cites NB500-319

Palchaudhuri R, Saez B, Hoggatt J et al. Non-genotoxic conditioning for hematopoietic stem cell transplantation using a hematopoietic-cell-specific internalizing immunotoxin. Nat

Cites ab8216

Nel I  et al. Circulating Tumor Cell Composition in Renal Cell Carcinoma. PLoS One 11:e0153018 (2016).

Cites ab8216

Nel I  et al. Individual profiling of circulating tumor cell composition in patients with non-small cell lung cancer receiving platinum based treatment. Transl Lung Cancer Res 3:100-6 (2014).

Cites ab8216

Thakolwiboon S  et al. Heterogeneity of The CD90(+) Population in Different Stages of Hepatocarcinogenesis. J Proteomics Bioinform 7:296-302 (2014).

Cites ab8216

Weller P  et al. Detection of circulating tumor cell subpopulations in patients with head and neck squamous cell carcinoma (HNSCC). PLoS One 9:e113706 (2014).

Cites ab8216

Lunardi S  et al. IP-10/CXCL10 induction in human pancreatic cancer stroma influences lymphocytes recruitment and correlates with poor survival. Oncotarget 5:11064-80 (2014).

Cites ab8216

Bazil V  et al. Sialic acid-dependent epitopes of CD45 molecules of restricted cellular expression. Immunogenetics 29:202-5 (1989).

Cites ab8216

Smith AM  et al. Adult human glia, pericytes and meningeal fibroblasts respond similarly to IFNy but not to TGFß1 or M-CSF. PLoS One 8:e80463 (2013).

Cites NB500-319

Dai J, Zhang H, Karatsinides A et al. Cabozantinib inhibits prostate cancer growth and prevents tumor-induced bone lesions. Clin Cancer Res 2013 Oct 4

Cites ab8216

Nel I  et al. Formation and repair kinetics of Pt-(GpG) DNA adducts in extracted circulating tumour cells and response to platinum treatment. Br J Cancer 109:1223-9 (2013).

Cites ab8216

Nel I  et al. Individual profiling of circulating tumor cell composition and therapeutic outcome in patients with hepatocellular carcinoma. Transl Oncol 6:420-8 (2013).

Cites ab8216

Smith AM  et al. M-CSF increases proliferation and phagocytosis while modulating receptor and transcription factor expression in adult human microglia. J Neuroinflammation 10:85 (2013).

Cites ab8216

Ara T  et al. Critical role of STAT3 in IL-6-mediated drug resistance in human neuroblastoma. Cancer Res 73:3852-64 (2013).

Cites ab8216

Smith AM  et al. The transcription factor PU.1 is critical for viability and function of human brain microglia. Glia 61:929-42 (2013).

Cites NB500-319

Koethe S, Zander L, Koster S et al. CD45RB glycosylation is specifically regulated during human peripheral B cell differentiation J Leukoc Biol 2011 Jul

Cites ab8216

Raymond AD  et al. HIV Type 1 Nef is released from infected cells in CD45(+) microvesicles and is present in the plasma of HIV-infected individuals. AIDS Res Hum Retroviruses 27:167-78 (2011).

Cites ab8216

Ali SA  et al. Genetic characterization of HIV type 1 Nef-induced vesicle secretion. AIDS Res Hum Retroviruses 26:173-92 (2010).

Cites ab8216

Goodman WA  et al. IL-6 signaling in psoriasis prevents immune suppression by regulatory T cells. J Immunol 183:3170-6 (2009).

Cites ab8216

Thorogate R  et al. A novel fluorescence-based method in forensic science for the detection of blood in situ. Forensic Sci Int Genet 2:363-71 (2008).

Cites ab8216

Kirchberger S  et al. The cytoplasmic tail of CD45 is released from activated phagocytes and can act as an inhibitory messenger for T cells. Blood 112:1240-8 (2008).

Cites NB500-319

Drbal K, Moertelmaier M, Holzhauser C et al. Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement Int Immunol 2007 May

Cites NB500-319

Cermak L, Simova S, Pintzas A et al. Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells; Roles of transcription Daxx expression, and adhesion molecules J Biol Chem 2002 Mar 8

Cites NB500-319

Ilangumaran S, Briol A, Hoessli DC. CD44 selectively associates with active Src family protein tyrosine kinases Lck and Fyn in glycosphingolipid-rich plasma membrane domains of human peripheral blood lymphocytes Blood Details:This publication used the Allophycocyanin conjugated form of this antibody (Cat# NB500-633).

Cites NB500-319

Cermak L, Simova S, Pintzas A et al. Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells; Roles of transcription Daxx expression, and adhesion molecules J Biol Chem Details:This publication used the Allophycocyanin conjugated form of this antibody (Cat# NB500-633).

Cites NB500-319

Drbal K, Moertelmaier M, Holzhauser C et al. Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement Int Immunol Details:This publication used the Allophycocyanin conjugated form of this antibody (Cat# NB500-633).

Cites NB500-319

Koethe S, Zander L, Koster S et al. CD45RB glycosylation is specifically regulated during human peripheral B cell differentiation J Leukoc Biol Details:This publication used the Allophycocyanin conjugated form of this antibody (Cat# NB500-633).

Cites NB500-319

Horejsi V, Angelisova P, Bazil V et al. Monoclonal antibodies against human leucocyte antigens II Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74) Folia Biol (Praha) Jun 10 1905 Details:This publication used the Allophycocyanin conjugated form of this antibody (Cat# NB500-633).

Rating

Species

Application

Date

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Human

IHC-P

18-Dec-2012


Total Citations (5)
Description: Mouse monoclonal (2D1) antibody to CD45.
Application: FC
Host: Mouse
Isotype: IgG1
Reactivity: Human

£176





Total Citations (1)
Description: Rabbit monoclonal (E19-G) antibody to CD45.
Application: IHC-P, IHC-Fr
Host: Rabbit
Isotype: IgG
Reactivity: Human

£192 – £515

Description: Rabbit polyclonal antibody to PTPRC.
Application: WB, IHC
Host: Rabbit
Isotype: IgG
Reactivity: Human, Mouse, Rat

£148 – £372

Description: Rabbit polyclonal antibody to CD45 (Phospho-Ser1007)
Application: WB
Host: Rabbit
Reactivity: Human
Immunogen: Peptide sequence around phosphorylation site of serine 1007 (D-D-S(p)-D-S) derived from Human CD45.

£180 – £234

Please Note: Anti-CD45 Antibody is for research use only. It is not intended for diagnostic or therapeutic use.
Antibodies.com | A85889
Size Price Quantity
100µg £176

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