Anti-CD62L Antibody [MEL-14] (A281091)

Anti-CD62L Antibody [MEL-14]

Rat monoclonal [MEL-14] antibody to CD62L.

This antibody recognises mouse L-selectin. L-selectin is an adhesion molecule expressed on most T and B lymphocytes, neutrophils, monocytes and eosinophils, (Fink, P. et al., 1985).Pre-incubation of lymphocytes with MEL-14 completely and specifically blocks binding of lymphocytes to high endothelial venules (HEV) in vitro and the migration of lymphocytes to lymph nodes in vivo (Gallatin, W. et al. 1983). Removal of sodium azide is recommended prior to using this clone in functional assays.

Flow Cytometry, IHC-Fr, IP

Flow Cytometry: 1 µg/ml, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Mouse

Mouse B cell lymphoma, 38C-14.

Rat

Monoclonal

MEL-14

IgG2a

Unconjugated

Protein G affinity chromatography of ascites.

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rat anti mouse CD62L, clone MEL-14, recognizes mouse L-selectin. L-selectin is an adhesion molecule expressed on most T and B lymphocytes, neutrophils, monocytes and eosinophils, (Fink, P. et al., 1985).Pre-incubation of lymphocytes with MEL-14 completely and specifically blocks binding of lymphocytes to high endothelial venules (HEV) in vitro and the migration of lymphocytes to lymph nodes in vivo (Gallatin, W. et al. 1983). Removal of sodium azide is recommended prior to using this clone in functional assays.

A.11 , AI528707, CD62 antigen ligand , CD62 antigen-like family member L, gp90 MEL , gp90-MEL, hLHRc, IgA nephropathy, susceptibility to, included , L Selectin , L-selectin, LAM-1, LAM1 , LECAM1 , Leu8, Leukocyte adhesion molecule 1 , Leukocyte surface antigen Leu-8, Leukocyte surface antigen Leu8 , Leukocyte-endothelial cell adhesion molecule 1 , LNHR, LSEL, Ly-22 , Ly-m22 , Lyam-1, LYAM1 , LYAM1_HUMAN, Lymph node homing receptor , Lymphocyte adhesion molecule 1, Lymphocyte antigen 22 , Lymphocyte surface MEL-14 antigen , MEL-14, Pln homing receptor, PLNHR , Selectin L , Selectin, lymphocyte , SELL, TQ1