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Anti-CD8 alpha Antibody [ARC59319] (A309962)

Western Blot - Anti-CD8 alpha Antibody [ARC59319] (A309962) - Antibodies.com Immunohistochemistry - Anti-CD8 alpha Antibody [ARC59319] (A309962) - Antibodies.com Immunohistochemistry - Anti-CD8 alpha Antibody [ARC59319] (A309962) - Antibodies.com Immunofluorescence - Anti-CD8 alpha Antibody [ARC59319] (A309962) - Antibodies.com
This product is discontinued

Anti-CD8 alpha Antibody [ARC59319] (A309962) has been discontinued and is no longer available.

View all Anti-CD8 alpha Antibodies.

Name
Anti-CD8 alpha Antibody [ARC59319]
Description
Rabbit monoclonal [ARC59319] antibody to CD8 alpha.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:1,000-1:5,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivity
Mouse
Immunogen
Recombinant protein of mouse CD8A.
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC59319
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
35 - 42 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
alpha polypeptide (p32), CD8, CD8 antigen alpha polypeptide, CD8 antigen alpha polypeptide (p32), CD8a, CD8A antigen, CD8A molecule, CD8A_HUMAN, Leu2, Leu2 T lymphocyte antigen, Ly3, LYT3, MAL, OKT8 T cell antigen, OTTHUMP00000160760, OTTHUMP00000160764, OTTHUMP00000203528, OTTHUMP00000203721, p32, T cell antigen Leu2, T cell co receptor, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, T8 T cell antigen, T8/Leu-2 T-lymphocyte differentiation antigen
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-CD8 alpha Antibody [ARC59319] (A309962) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-CD8 alpha Antibody [ARC59319] (A309962)
Western blot analysis of various lysates, using Anti-CD8 alpha Antibody [ARC59319] (A309962) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry - Anti-CD8 alpha Antibody [ARC59319] (A309962) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-CD8 alpha Antibody [ARC59319] (A309962)
Immunohistochemistry analysis of paraffin-embedded mouse colon using Anti-CD8 alpha Antibody [ARC59319] (A309962) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-CD8 alpha Antibody [ARC59319] (A309962) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-CD8 alpha Antibody [ARC59319] (A309962)
Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-CD8 alpha Antibody [ARC59319] (A309962) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence - Anti-CD8 alpha Antibody [ARC59319] (A309962) - Antibodies.comEnlarge Image
Figure 4: Immunofluorescence - Anti-CD8 alpha Antibody [ARC59319] (A309962)
Immunofluorescence analysis of mouse thymus using Anti-CD8 alpha Antibody [ARC59319] (A309962) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Publishing research using Anti-CD8 alpha Antibody [ARC59319] (A309962)? Please let us know so that we can list the citation on this page.

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