Anti-CD8 alpha Antibody [OX-8] - BSA and Azide free (A281501)

$800

Mouse monoclonal [OX-8] antibody to CD8 alpha for IHC-Fr, IHC-P, WB, Flow Cytometry, IP and Functional Assays.

Alternative Formats and ConjugatesAlternative Formats

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-CD8 alpha Antibody [OX-8] - BSA and Azide free
Description
Mouse monoclonal [OX-8] antibody to CD8 alpha.
Specificity
This antibody recognises the rat CD8 alpha cell surface antigen, expressed by a subset of T lymphocytes, most thymocytes and the majority of NK cells. Mouse anti Rat CD8a, clone MRC OX-8 is suitable for use in in vitro blocking assays. Mouse anti Rat CD8a, clone MRC OX-8 reacts with paraffin-embedded material following PLP Fixation (periodate-lysine paraformaldehyde). Mouse anti Rat CD8a, clone MRC OX-8 is routinely tested in flow cytometry on rat splenocytes.
Applications
IHC-Fr, IHC-P, WB, Flow Cytometry, IP, Functional Assays
Dilutions
Flow Cytometry: 1:50 - 1:100, Use 10µl of the suggested working dilution to label 106 cells in 100µl. This product does not require protein digestion pre-treatment of paraffin embedded sections.This product does not require antigen retrieval using heat treatment prior to staining of paraffin embedded sections.
Reactivity
Rat
Immunogen
Rat Thymocyte Membrane Glycoproteins.
Host
Mouse
Clonality
Monoclonal
Clone ID
OX-8
Isotype
IgG1
Conjugate

Unconjugated

Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline.
Storage
Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Mouse anti Rat CD8a, clone MRC OX-8, recognizes the rat CD8 alpha cell surface antigen, expressed by a subset of T lymphocytes, most thymocytes and the majority of NK cells.Mouse anti Rat CD8a, clone MRC OX-8 is suitable for use in in vitro blocking assays (Popov et al.2001).Mouse anti Rat CD8a, clone MRC OX-8 reacts with paraffin-embedded material following PLP Fixation (periodate-lysine paraformaldehyde) (Whiteland et al. 1995).Mouse anti Rat CD8a, clone MRC OX-8 is routinely tested in flow cytometry on rat splenocytes.
Synonyms
alpha polypeptide (p32), CD8, CD8 antigen alpha polypeptide, CD8 antigen alpha polypeptide (p32), CD8a, CD8A antigen, CD8A molecule, CD8A_HUMAN, Leu2, Leu2 T lymphocyte antigen, Ly3, LYT3, MAL, OKT8 T cell antigen, OTTHUMP00000160760, OTTHUMP00000160764, OTTHUMP00000203528, OTTHUMP00000203721, p32, T cell antigen Leu2, T cell co receptor, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, T8 T cell antigen, T8/Leu-2 T-lymphocyte differentiation antigen
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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