Western Blot - Anti-CDK1 Antibody [Assigned #A0030] (A16766)
Western blot analysis of extracts of various cell lines, using Anti-CDK1 Antibody (A0030) at 1:100 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.