The antibody detects endogenous level of Myc only when phosphorylated at threonine 58.
Applications
WB, IHC
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of threonine 58 (L-P-T(p)-P-P) derived from Human Myc.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
BHLHE39, Class E basic helix-loop-helix protein 39, Myc proto-oncogene protein, Proto-oncogene c-Myc, Transcription factor p64
Western blot analysis of extracts from Hela cells using Myc (Phospho-Thr58) Antibody #11034 (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Myc (Phospho-Thr58) Antibody #11034 (left) or the same antibody preincubated with blocking peptide (right).
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