Rabbit polyclonal antibody to STAT3 (Phospho-Tyr705)
Specificity
The antibody detects endogenous level of STAT3 only when phosphorylated at tyrosine 705.
Applications
WB, IHC, ICC/IF, IP
Dilutions
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of tyrosine 705 (A-P-Y(p)-L-K) derived from Human STAT3.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Predicted MW
88kd
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Acute-phase response factor, APRF, Signal transducer and activator of transcription 3
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT3 (Phospho-Tyr705) Antibody #11045 (left) or the same antibody preincubated with blocking peptide #51045 (right).
