Rabbit polyclonal antibody to 4E-BP1 (phospho Thr70).
Spezifität
This antibody detects endogenous levels of 4E-BP1 only when phosphorylated at Thr70.
Anwendungen
IHC, ELISA
Verdünnungen
WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:5000
Reaktivität
Human
Immunogen
Synthetic peptide derived from human 4E-BP1 around the phosphorylation site of Thr70 (amino acids 36-85).
Wirt
Rabbit
Klonalität
Polyclonal
Isotyp
IgG
Konjugat
Unconjugated
Reinigung
Purified from rabbit serum by antigen affinity chromatography using the immunizing peptide.
Molekulargewicht
12kDa
Produktform
Liquid
Formulierung
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyme
4E-BP1, 4EBP1, 4EBP1_HUMAN, BP 1, eIF4E binding protein 1, eIF4E-binding protein 1, Eukaryotic translation initiation factor 4E-binding protein 1, PHAS-I, PHASI, Phosphorylated heat- and acid-stable protein regulated by insulin 1
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Anti-4E-BP1 (phospho Thr70) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
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