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Independent Antibody Comparison

A comparison of identical primary antibodies from Abcam, Antibodies.com, Novus Biologicals, Sigma Aldrich, and Thermo Fisher.

An independent comparison of identical Anti-AIF1 Antibodies, Anti-GFAP Antibodies, and Anti-Vimentin Antibodies, that are commercially available from Abcam, Antibodies.com, Novus Biologicals, Sigma Aldrich, and Thermo Fisher. All of the antibodies compared are identical and originate from the same primary manufacturers. The antibody testing was performed by an independent laboratory and the slight differences in performance between some of the antibodies is likely due to different lots being tested and / or the different storage histories of each vial.

Anti-GFAP Antibodies

Identical Anti-GFAP Antibodies from Abcam, Antibodies.com, Novus Biologicals, and Thermo Fisher were used for immunofluorescent analysis of paraformaldehyde-fixed U251 cells and western blot analysis of rat brain lysate.

Supplier Product Code Lot Size List Price
Abcam ab7260 GR3287463-4 50µl $675
Antibodies.com A85419 X218 50µl $380
Novus Biologicals NB300-141 179-031817 50µl $429
Thermo Fisher PA3-16727 UJ2864104 50µl $483

Anti-AIF1 Antibodies

Identical Anti-AIF1 Antibodies from Abcam, Antibodies.com, Novus Biologicals, and Sigma Aldrich were used for immunofluorescent analysis of paraformaldehyde-fixed U937 cells and western blot analysis of mouse brain lysate.

Supplier Product Code Lot Size List Price
Abcam ab5076 GR3322398-1 100µg $680
Antibodies.com A82670 X86 100µg $455
Novus Biologicals NB100-1028 S7C7G2P41 E131219 100µg $479
Sigma Aldrich SAB2500041 5419S7G2P25 100µg $545

Anti-Vimentin Antibodies

Identical Anti-Vimentin Antibodies [VI-10] from Abcam, Antibodies.com, Novus Biologicals, and Thermo Fisher were used for immunofluorescent analysis of paraformaldehyde-fixed K562 cells and western blot analysis of HeLa cell lysate, HEK293 cell lysate, and Jurkat cell lysate.

Supplier Product Code Lot Size List Price
Abcam ab20346 GR3230520-18 100µg $630
Antibodies.com A86652 X84 100µg $345
Novus Biologicals NB500-563 530982 100µg $349
Thermo Fisher MA1-10459 530982 100µg $400

* It is interesting to note the identical lot numbers above from Novus Biologicals and Thermo Fisher; rather than reassigning their own lot numbers they have reused the lot number from the primary manufacturer. All online list prices taken in January 2023.

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