This alpha Ketoglutarate Dehydrogenase Assay Kit is a quantitative assay kit for measuring alpha Ketoglutarate Dehydrogenase activity in animal and plant tissues, and cells.
Anwendungen
Functional Studies
Assay-Prinzip
This kit provides a simple, sensitive and rapid alpha-ketoglutarate dehydrogenase activity detection method, which is compatible with various biological samples, especially animals or plant tissues, and cells. The detection principle is based on the fact that alpha-ketoglutarate dehydrogenase can catalyze the conversion of alpha-ketoglutarate, NAD+ and coenzyme A to produce succinyl-coenzyme A, carbon dioxide and NADH. NADH has a characteristic absorption peak at 340 nm. Alpha-ketoglutarate dehydrogenase activity is calculated by measuring the rate of formation of NADH.
Probentyp
Animal tissues, plant tissues, and cells.
Komponenten
Extraction Buffer, Reagent I, Reagent II, Reagent III, Reagent IV, Reagent V, Reagent VI, Reagent VII, Reagent VIII
Lagerung
Shipped at 4°C. Refer to the Protocol Manual for storage conditions of individual components. Do not use past expiration date!
Allgemeine Hinweise
96-well UV microplate / microquartz cuvette is required.
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.
