Figure 1: Western Blot - Anti-ATG12 Antibody [ARC2663] (A309284)
Western blot analysis of various lysates, using Anti-ATG12 Antibody [ARC2663] (A309284) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded mouse lung using Anti-ATG12 Antibody [ARC2663] (A309284) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Figure 3: Western Blot - Anti-ATG12 Antibody [ARC2663] (A309284)
Immunoprecipitation analysis of 300µg extracts of 293T cells using 3µg of Anti-ATG12 Antibody [ARC2663] (A309284). This Western blot was performed on the immunoprecipitate using Anti-ATG12 Antibody [ARC2663] (A309284) at a dilution of 1:500.
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