Anti-ATG4B Antikörper (A304916)

Name: Anti-ATG4B Antibody
Brand: Antibodies.com
SKU: A304916
Price: 500.0 USD
Availability: InStock

Bild Vergrößern

$500

Polyklonal Kaninchen Antikörper gegen ATG4B für WB und ICC/IF.

100% Qualitätsgarantie

Preis-Match-Garantie

Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

Garantien

100% Qualitätsgarantie

Preis-Match-Garantie

Produktdetails

Produktname

Anti-ATG4B Antibody

Beschreibung

Rabbit polyclonal antibody to ATG4B.

Anwendungen

WB, ICC/IF

Verdünnungen

WB: 1:1,000, ICC/IF: 1:100

Reaktivität

Human, Mouse

Immunogen

Synthetic peptide from the N-terminal of Human ATG4B (aa. 62-73).

Wirt

Rabbit

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Peptide affinity purification.

Konzentration

1 mg/ml

Molekulargewicht

~50 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.09% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

APG4 autophagy 4 homolog B, APG4B, ATG4 autophagy related 4 homolog B (S. cerevisiae), ATG4B_HUMAN, AUT like 1 cysteine endopeptidase, AUT-like 1 cysteine endopeptidase, AUTL1, Autophagin 1, Autophagin-1, Autophagy related 4B cysteine peptidase, Autophagy related cysteine endopeptidase 1, Autophagy related protein 4 homolog B, Autophagy-related cysteine endopeptidase 1, Autophagy-related protein 4 homolog B, Cysteine protease ATG4B, hAPG4B, MGC1353

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (2)

Bild Vergrößern

Figure 1: Immunofluorescence - Anti-ATG4B Antibody (A304916)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-ATG4B Antibody (A304916), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:200 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Cytoplasm. Magnification: 60X.(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4B Antibody (D) Composite.

Bild Vergrößern

Figure 2: Western Blot - Anti-ATG4B Antibody (A304916)

Western blot analysis of mouse brain cell lysates showing detection of 44.3 kDa ATG4B protein using Anti-ATG4B Antibody (A304916) at 1:1,000 for 1 hour at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: mouse brain cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: 44.3 kDa.