Anti-Aurora A + B + C Kinase Antikörper [4A7] (A85401)

Name: Anti-Aurora A + B + C Kinase Antibody [4A7]
Brand: Antibodies.com
SKU: A85401
Price: 185.0 USD
Availability: InStock

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$185

Monoklonal [4A7] Maus Antikörper gegen Aurora A für WB, ICC/IF und IHC.

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Versandkosten

$40

Vorlaufzeit

Lieferung in 4-6 Werktagen

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+1 (314) 370-6046

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Produktdetails

Produktname

Anti-Aurora A + B + C Kinase Antibody [4A7]

Beschreibung

Mouse monoclonal (4A7) antibody to Aurora A + B + C Kinase.

Spezifität

This antibody was raised against human Aurora A protein and was shown to also bind Aurora B and Aurora C; as a result the epitope is likely within the highly conserved serine/threonine kinase domain. This antibody can be used to identify dividing or soon to be dividing cells and is also an excellent marker of midbodies both during and after cell division.

Anwendungen

WB, ICC/IF, IHC

Verdünnungen

WB: 1:1,000, ICC/IF: 1:1,000-1:2,000, IHC: 1:1,000-1:2,000

Reaktivität

Human, Horse, Bovine, Porcine, Rat, Mouse

Immunogen

Recombinant full-length human Aurora A protein, expressed in and purified from E. coli.

Wirt

Mouse

Klonalität

Monoclonal

Klon

4A7

Isotyp

IgG1

Konjugat

Unconjugated

Reinigung

Immunogen affinity purification.

Konzentration

1 mg/ml

Molekulargewicht

46kDa, 38kDa, 35kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline with 50% Glycerol and 5mM Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

AIK, ARK-1, ARK1, AURA, Aurka, Aurora 2, Aurora kinase A, Aurora-related kinase 1, Aurora/IPL1 like kinase, Aurora/IPL1-related kinase 1, AURORA2, Breast tumor-amplified kinase, BTAK, hARK1, IAK, IPL1 related kinase, MGC34538, OTTHUMP00000031340, OTTHUMP00000031341, OTTHUMP00000031342, OTTHUMP00000031343, OTTHUMP00000031344, OTTHUMP00000031345, OTTHUMP00000166071, OTTHUMP00000166072, PPP1R47, Protein phosphatase 1, regulatory subunit 47, Serine/threonine kinase 15, Serine/threonine kinase 6, Serine/threonine-protein kinase 15, Serine/threonine-protein kinase 6, Serine/threonine-protein kinase aurora-A, STK15, STK6, STK6_HUMAN, STK7

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (2)

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Figure 1: Immunofluorescence - Anti-Aurora A + B + C Kinase Antibody [4A7] (A85401)

HeLa cell cultures were stained with Anti-Aurora A/B/C Kinase Antibody (green). In this image, strong staining in spindle poles was seen in cells at anaphase. This antibody also stains the midbodies between the two daughter cells. Cells were counterstained with Anti-Vimentin Antibody (A85421 | red). Blue is a DNA stain.

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Figure 2: Western Blot - Anti-Aurora A + B + C Kinase Antibody [4A7] (A85401)

Left: Western blot analysis of Anti-Aurora A/B/C Kinase Antibody in HeLa cells. Blot of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with Anti-Aurora A/B/C Kinase Antibody. Nocodazole is a microtubule depolymerizating agent which induces cells to halt at the G2/M phase and also induces Aurora kinase expression. The Anti-Aurora A/B/C Kinase Antibody binds strongly to bands at about 46 kDa and 38kDa, corresponding to Aurora A and Aurora B. Also, it recognizes a weak band at 35 kDa which is Aurora C. Right: Blot of recombinant full length human Aurora A, B and C was probed with Anti-Aurora A/B/C Kinase Antibody. This antibody reacts strongly with all three Aurora kinases proteins.