The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Plattform

Microplate

Nachweismethode

Colorimetric

Probentyp

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Sensitivität

1.56 ng/ml

Detektionsbereich

6.25 - 400 ng/ml

Testdauer

1 - 4.5 Hours

Reaktivität

Bovine

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Synonyms

Angiopoietin 1 receptor, Angiopoietin-1 receptor, CD202b, CD202b antigen, EC 2.7.10.1, Endothelial tyrosine kinase, Endothelium specific receptor tyrosine kinase 2, hTIE 2, hTIE2, Hyk, p140 TEK, soluble TIE2 variant 1, soluble TIE2 variant 2, Tek, tek tyrosine kinase, TEK tyrosine kinase endothelial, TIE 2, TIE2, TIE2_HUMAN, Tunica interna endothelial cell kinase, Tyrosine kinase with Ig and EGF homology domains 2, Tyrosine kinase with Ig and EGF homology domains-2, Tyrosine protein kinase receptor TEK, Tyrosine protein kinase receptor TIE 2, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE 2, Tyrosine-protein kinase receptor TIE-2, Venous malformations multiple cutaneous and mucosal, venous malformations, multiple cutaneous and mucosal, VMCM, VMCM 1, VMCM1

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.