Anti-Caspase-3 Antikörper (A308662)

Name: Anti-Caspase-3 Antibody
Brand: Antibodies.com
SKU: A308662
Price: 470.0 USD
Availability: InStock

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$470

Polyklonal Kaninchen Antikörper gegen Caspase-3 für WB, IHC und ICC/IF.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

Garantien

100% Qualitätsgarantie

Preis-Match-Garantie

Stichworte:

Knockout-validierter Antikörper

Produktdetails

Produktname

Anti-Caspase-3 Antibody

Beschreibung

Rabbit polyclonal antibody to Caspase-3.

Anwendungen

WB, IHC, ICC/IF

Verdünnungen

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reaktivität

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 29-175 of human Caspase-3 (NP_004337.2).

Sequenz

SGISLDNSYKMDYPEMGLCIIINNKNFHKSTGMTSRSGTDVDAANLRETFRNLKYEVRNKNDLTREEIVELMRDVSKEDHSKRSSFVCVLLSHGEEGIIFGTNGPVDLKKITNFFRGDRCRSLTGKPKLFIIQACRGTELDCGIETD

Wirt

Rabbit

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity purification.

Molekulargewicht

32 kDa / 17 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

A830040C14Rik, Apopain, CASP-3, CASP3, Casp3a, CASP3_HUMAN, Caspase 3, Caspase 3, apoptosis-related cysteine peptidase, Caspase 3, apoptosis-related cysteine protease, Caspase 3, apoptosis-related cysteine protease a, Caspase-3 subunit p12, CC3, CPP-32, CPP32, CPP32B, Cysteine protease CPP32, EC 3.4.22.56, LICE, mldy, OTTHUMP00000165052, OTTHUMP00000165053, OTTHUMP00000165054, PARP cleavage protease, Procaspase3, Protein Yama, SCA 1, SCA-1, SREBP cleavage activity 1, Yama

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (9)

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Figure 1: Western Blot - Anti-Caspase-3 Antibody (A308662)

Western blot analysis of extracts of Mouse lung, using Anti-Caspase-3 Antibody (A308662) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-Caspase-3 Antibody (A308662)

Western blot analysis of extracts of Jurkat cells, using Anti-Caspase-3 Antibody (A308662) at 1:1,000 dilution. Jurkat cells were treated by staurosporine(1 uM) for 3 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 3: Western Blot - Anti-Caspase-3 Antibody (A308662)

Western blot analysis of extracts from wild type(WT) and Caspase-3 p17 knockout (KO) 293T cells, using Anti-Caspase-3 Antibody (A308662) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 4: Immunohistochemistry - Anti-Caspase-3 Antibody (A308662)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-Caspase-3 Antibody (A308662) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.