This Catalase Activity Assay Kit is a quantitative assay kit for measuring Catalase activity in serum, plasma, animal and plant tissues and cells.
Anwendungen
Functional Studies
Assay-Prinzip
This kit provides a simple and easy colorimetric assay for the study of catalase activity in a variety of biological samples such as cell and tissue lysates or biological fluids. This assay kit utilizes the peroxidase function of catalase for measuring catalase activity.In ther presence of an appropriate concentration of hydrogen peroxide, catalase reacts with methanol to produce formaldehyde which can react with chromogens. The formaldehyde produced can be measured colorimetrically at OD 540 nm.
Probentyp
Serum, plasma, animal tissues, plant tissues, and cells.
Komponenten
Assay Buffer, Sample Diluent, Formaldehyde Standard (4.25 M), Catalase (Control), Potassium Hydroxide, Hydrogen Peroxide, Chromogen, Potassium Periodate
Lagerung
Shipped at 4°C. Store at -20°C in the dark. Refer to the Protocol Manual for storage conditions of individual components. Do not use past expiration date!
Allgemeine Hinweise
96-well microplate / microglass cuvette is required.
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.
Figure 1: Standard Curve - Catalase Activity Assay Kit (A319666)
Representative formaldehyde standard curve for Catalase Activity Assay Kit (A319666). The y-axis is absorbance of standards and the x-axis is final formaldehyde concentration (µM).
