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Anti-CD146 Antikörper (A88042)

Western Blot - Anti-CD146 Antibody (A88042) - Antibodies.com Western Blot - Anti-CD146 Antibody (A88042) - Antibodies.com Immunohistochemistry - Anti-CD146 Antibody (A88042) - Antibodies.com Immunohistochemistry - Anti-CD146 Antibody (A88042) - Antibodies.com Immunohistochemistry - Anti-CD146 Antibody (A88042) - Antibodies.com Immunofluorescence - Anti-CD146 Antibody (A88042) - Antibodies.com Immunofluorescence - Anti-CD146 Antibody (A88042) - Antibodies.com
$335
Product Datasheet

Polyklonal Kaninchen Antikörper gegen CD146 für WB, IHC und ICC/IF.

100% Qualitätsgarantie
Preis-Match-Garantie

Versandkosten
$40
Vorlaufzeit
Lieferung in 5-8 Werktagen

Telefon
+1 (314) 370-6046
Mo bis Frei, 8am - 4pm AST
Email
orders@antibodies.com

Produktname
Anti-CD146 Antibody
Beschreibung
Rabbit polyclonal antibody to CD146.
Anwendungen
WB, IHC, ICC/IF
Verdünnungen
WB: 1:100-1:500, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reaktivität
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 24-559 of human CD146/MCAM (NP_006491.2).
Sequenz
VPGEAEQPAPELVEVEVGSTALLKCGLSQSQGNLSHVDWFSVHKEKRTLIFRVRQGQGQSEPGEYEQRLSLQDRGATLALTQVTPQDERIFLCQGKRPRSQEYRIQLRVYKAPEEPNIQVNPLGIPVNSKEPEEVATCVGRNGYPIPQVIWYKNGRPLKEEKNRVHIQSSQTVESSGLYTLQSILKAQLVKEDKDAQFYCELNYRLPSGNHMKESREVTVPVFYPTEKVWLEVEPVGMLKEGDRVEIRCLADGNPPPHFSISKQNPSTREAEEETTNDNGVLVLEPARKEHSGRYECQGLDLDTMISLLSEPQELLVNYVSDVRVSPAAPERQEGSSLTLTCEAESSQDLEFQWLREETGQVLERGPVLQLHDLKREAGGGYRCVASVPSIPGLNRTQLVNVAIFGPPWMAFKERKVWVKENMVLNLSCEASGHPRPTISWNVNGTASEQDQDPQRVLSTLNVLVTPELLETGVECTASNDLGKNTSILFLELVNLTTLTPDSNTTTGLSTSTASPHTRANSTSTERKLPEPESRG
Wirt
Rabbit
Klonalität
Polyclonal
Isotyp
IgG
Konjugat

Unconjugated

Reinigung
Affinity purification.
Molekulargewicht
120 kDa
Produktform
Liquid
Formulierung
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyme
A32 antigen, CD 146, CD146 antigen, Cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12, Gicerin, Mcam, Melanoma adhesion molecule, Melanoma associated antigen A32, Melanoma associated antigen MUC18, Melanoma associated glycoprotein MUC18, Melanoma cell adhesion molecule, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, MelCAM, MUC 18, MUC18, MUC18_HUMAN, S endo 1, S endo 1 endothelial associated antigen, S-endo 1 endothelial-associated antigen
Isotyp-Kontrollantikörper
Kompatible Sekundärantikörper
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.
Western Blot - Anti-CD146 Antibody (A88042) - Antibodies.comBild Vergrößern
Figure 1: Western Blot - Anti-CD146 Antibody (A88042)
Western blot analysis of extracts of various cell lines, using Anti-CD146 Antibody (A88042) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Western Blot - Anti-CD146 Antibody (A88042) - Antibodies.comBild Vergrößern
Figure 2: Western Blot - Anti-CD146 Antibody (A88042)
Western blot analysis of extracts of various cell lines, using Anti-CD146 Antibody (A88042) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
Immunohistochemistry - Anti-CD146 Antibody (A88042) - Antibodies.comBild Vergrößern
Figure 3: Immunohistochemistry - Anti-CD146 Antibody (A88042)
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-CD146 Antibody (A88042) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-CD146 Antibody (A88042) - Antibodies.comBild Vergrößern
Figure 4: Immunohistochemistry - Anti-CD146 Antibody (A88042)
Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-CD146 Antibody (A88042) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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