This antibody recognises the human CD151 cell surface antigen, also known as PETA-3. CD151 is expressed by epithelial cells, endothelial cells, platelets, megakaryocytes, monocytes and in the renal glomeruli and proximal and distal tubules. CD151 is not expressed by lymphocytes or granulocytes. More recently CD151 has also been shown to be expressed by erythrocytes, and to carry the MER2 blood group antigen. It should be noted that CD151 is very closely associated with the alpha3 beta1 integrin in cells and co-immunoprecipitation may occur even in quite stringent conditions (Yauch. et al. 1998).

Anwendungen

WB, IHC-P, IP, Flow Cytometry, IHC-Fr, ELISA, IF

Verdünnungen

Flow Cytometry: 1:50 - 1:100, IHC-P: 1:100, Use 10µl of the suggested working dilution to label 10⁶ cells or 100µl whole blood

Reaktivität

Human

Wirt

Mouse

Klonalität

Monoclonal

Klon

11G5a

Isotyp

IgG1

Konjugat

Unconjugated

Reinigung

Protein G affinity chromatography of tissue culture supernatant.

Konzentration

1 mg/ml

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Lagerung

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Allgemeine Hinweise

Mouse anti Human CD151 antibody, clone 11G5a recognizes the human CD151 cell surface antigen, also known as PETA-3. CD151 is expressed by epithelial cells, endothelial cells, platelets, megakaryocytes, monocytes and in the renal glomeruli and proximal and distal tubules. CD151 is not expressed by lymphocytes or granulocytes. More recently CD151 has also been shown to be expressed by erythrocytes, and to carry the MER2 blood group antigen (Crew et al. 2004).It should be noted that CD151 is very closely associated with the alpha3 beta1 integrin in cells and co-immunoprecipitation may occur even in quite stringent conditions (Yauch. et al. 1998).

Synonyme

CD151 antigen, CD151 molecule, CD151 molecule (Raph blood group), CD151_HUMAN, GP27, hemidesmosomal tetraspanin CD151, Membrane glycoprotein SFA-1, MER2, PETA-3, PETA3, Platelet endothelial tetraspan antigen 3, platelet surface glycoprotein gp27, Platelet-endothelial cell tetraspan antigen 3, Platelet-endothelial tetraspan antigen 3, RAPH, Red blood cell antigen MER2, SFA 1, SFA1, Tetraspanin-24, Tspan-24, TSPAN24

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.