Enoblituzumab Biosimilar - Anti-CD276 Antibody - BSA and Azide free
Beschreibung
Recombinant humanized monoclonal antibody to CD276.
Anwendungen
ELISA, Flow Cytometry
Verdünnungen
Flow Cytometry: 1:100
Reaktivität
Human
Wirt
Humanized
Klonalität
Monoclonal
Isotyp
IgG1
Konjugat
Unconjugated
Reinigung
Affinity chromatography.
Konzentration
Reconstitution dependent.
Produktform
Lyophilized
Rekonstitution
Reconstitute with distilled sterile water.
Formulierung
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Lagerung
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Allgemeine Hinweise
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
ELISA plate pre-coated by 2 µg/ml (100 µl/well) Recombinant Human CD276 Protein (Fc Chimera 6xHis Tag) (A318396) can bind Anti-CD276 Humanized Antibody [Enoblituzumab Biosimilar] - Azide free (A318946) in a linear range of 0.24-31.25 µg/ml.
HEK293 cell line transfected with irrelevant protein (B) and human B7-H3 (A) were surface stained with Anti-CD276 Humanized Antibody [Enoblituzumab Biosimilar] - Azide free (A318946) at 1 µg/ml followed by Anti-Human IgG Antibody (Alexa 488).
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