Anti-CDK2 (phospho Thr160) Antikörper [ARC57167] (A309005)

Name: Anti-CDK2 (phospho Thr160) Antibody [ARC57167]
Brand: Antibodies.com
SKU: A309005

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Dieses Produkt wurde eingestellt.

Anti-CDK2 (phospho Thr160) Antikörper [ARC57167] (A309005) wurde eingestellt und ist nicht mehr verfügbar.

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Produktdetails

Produktname

Anti-CDK2 (phospho Thr160) Antibody [ARC57167]

Beschreibung

Rabbit monoclonal [ARC57167] antibody to CDK2 (phospho Thr160).

Anwendungen

WB, IHC

Verdünnungen

WB: 1:2,000-1:6,000, IHC: 1:50-1:200

Reaktivität

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around T160 of human Phospho-CDK2-T160 (NP_001789.2).

Sequenz

TYTHE

Wirt

Rabbit

Klonalität

Monoclonal

Klon

ARC57167

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity purification.

Molekulargewicht

34 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

Cdc2 related protein kinase, cdc2-related protein kinase, CDC28, CDC2A, Cdk 2, CDK1, CDK2_HUMAN, CDKN2, Cell devision kinase 2, Cell division protein kinase 2, Cyclin dependent kinase 2, cyclin dependent kinase 2-alpha, Cyclin-dependent kinase 2, kinase Cdc2, MPF, p33 protein kinase, p33(CDK2)

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (3)

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Figure 1: Western Blot - Anti-CDK2 (phospho Thr160) Antibody [ARC57167] (A309005)

Western blot analysis of HeLa, using Anti-CDK2 (phospho Thr160) Antibody [ARC57167] (A309005) at 1:5,000 dilution. HeLa cells were treated by nocodazole (50 ng/ml) at 37°C for 20 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Western Blot - Anti-CDK2 (phospho Thr160) Antibody [ARC57167] (A309005)

Western blot analysis of C6, using Anti-CDK2 (phospho Thr160) Antibody [ARC57167] (A309005) at 1:5,000 dilution. C6 cells were treated by nocodazole (50 ng/ml) at 37°C for 20 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 3: Immunohistochemistry - Anti-CDK2 (phospho Thr160) Antibody [ARC57167] (A309005)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-CDK2 (phospho Thr160) Antibody [ARC57167] (A309005) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.