Anti-Chk1 Antikörper (A10183)

Name: Anti-Chk1 Antibody
Brand: Antibodies.com
SKU: A10183
Price: 335.0 USD
Availability: InStock

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$335

Polyklonal Kaninchen Antikörper gegen Chk1 für WB, IHC und ICC/IF.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

Garantien

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Polyklonal Kaninchen Antikörper gegen Chk1 für WB und ELISA.

Produktdetails

Produktname

Anti-Chk1 Antibody

Beschreibung

Rabbit polyclonal antibody to Chk1.

Anwendungen

WB, IHC, ICC/IF

Verdünnungen

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reaktivität

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 148-307 of Chk1 (NP_001265.2).

Sequenz

DFGLATVFRYNNRERLLNKMCGTLPYVAPELLKRREFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPLALLHKILVENPSARITIPDIKKDRWYNKPLKKGAKRPRVTSGGVSESPSGFSKHIQSNLDFSPVNSAS

Wirt

Rabbit

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity purification.

Molekulargewicht

56 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

C85740, Cell cycle checkpoint kinase, Checkpoint , S. pombe, homolog of, 1, Checkpoint kinase 1, Checkpoint kinase 1 homolog (S. pombe), CHEK 1, Chek1, Chk 1, CHK1 checkpoint homolog (S. pombe), CHK1_HUMAN, EC 2.7.11.1, rad27, Serine/threonine protein kinase Chk1, Serine/threonine-protein kinase CHK1, STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae)

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (7)

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Figure 1: Western Blot - Anti-Chk1 Antibody (A10183)

Western blot analysis of extracts of various cell lines, using Anti-Chk1 Antibody (A10183) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-Chk1 Antibody (A10183)

Western blot analysis of extracts of HT-29 cells, using Anti-Chk1 Antibody (A10183) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 3: Immunohistochemistry - Anti-Chk1 Antibody (A10183)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-Chk1 Antibody (A10183) at a dilution of 1:50 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Chk1 Antibody (A10183)

Immunohistochemistry analysis of paraffin-embedded rat spleen using Anti-Chk1 Antibody (A10183) at a dilution of 1:50 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.