Figure 1: Western Blot - Anti-Complement C3 alpha chain Antibody [2B5] (A85332)
Western blot analysis of Anti-a Subunit Of Complement Component 5 Antibody on human serum samples. 0.1 µg human C3 protein (Lane 1), 10 µg normal human serum proteins (Lane 2) was blotted with Anti-a Subunit Of Complement Component 5 Antibody (1:3,000). The Anti-a Subunit Of Complement Component 5 Antibody binds strongly and cleanly to a band at about 115 kDa which represents the intact a subunit of C3 and a weaker proteolytic band at approximately 45 kDa which is the C-terminal of the a subunit including the netrin domain. Bands at 190 kDa and above are likely the pro-C3 and its glycosylated form.
