Figure 1: Western Blot - Anti-Cyclin B1 Antibody [ARC0474] (A306720)
Western blot analysis of extracts from wild type (WT) and Cyclin B1 knockout (KO) HeLa cells, using Anti-Cyclin B1 Antibody [ARC0474] (A306720) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.
Figure 2: Western Blot - Anti-Cyclin B1 Antibody [ARC0474] (A306720)
Western blot analysis of extracts of various cell lines, using Anti-Cyclin B1 Antibody [ARC0474] (A306720) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.
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