Anti-Cyclin B1 Antikörper [ARC2614] (A309196)

Name: Anti-Cyclin B1 Antibody [ARC2614]
Brand: Antibodies.com
SKU: A309196

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Dieses Produkt wurde eingestellt.

Anti-Cyclin B1 Antikörper [ARC2614] (A309196) wurde eingestellt und ist nicht mehr verfügbar.

Alle Anti-Cyclin B1 Antikörper anzeigen.

Produktdetails

Produktname

Anti-Cyclin B1 Antibody [ARC2614]

Beschreibung

Rabbit monoclonal [ARC2614] antibody to Cyclin B1.

Anwendungen

WB, IHC, IP

Verdünnungen

WB: 1:500-1:1,000, IHC: 1:50-1:200, IP: 1:500-1:1,000

Reaktivität

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 334-433 of human Cyclin B1 (P14635).

Sequenz

YDMVHFPPSQIAAGAFCLALKILDNGEWTPTLQHYLSYTEESLLPVMQHLAKNVVMVNQGLTKHMTVKNKYATSKHAKISTLPQLNSALVQDLAKAVAKV

Wirt

Rabbit

Klonalität

Monoclonal

Klon

ARC2614

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity purification.

Molekulargewicht

55 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

CCNB, CCNB 1, ccnb1, CCNB1_HUMAN, G2 mitotic specific cyclin B1, G2/mitotic-specific cyclin-B1

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (3)

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Figure 1: Western Blot - Anti-Cyclin B1 Antibody [ARC2614] (A309196)

Western blot analysis of Jurkat, using Anti-Cyclin B1 Antibody [ARC2614] (A309196) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunohistochemistry - Anti-Cyclin B1 Antibody [ARC2614] (A309196)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Cyclin B1 Antibody [ARC2614] (A309196) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Western Blot - Anti-Cyclin B1 Antibody [ARC2614] (A309196)

Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-Cyclin B1 Antibody [ARC2614] (A309196). This Western blot was performed on the immunoprecipitate using Anti-Cyclin B1 Antibody [ARC2614] (A309196) at a dilution of 1:1000.