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Cyclin D1 (phospho Thr286) Cell Based Kit Elisa (A102528)

$645
Versandkosten
Vorlaufzeit
Lieferung in 5-8 Werktagen
Telefon
+1 (314) 370-6046
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Email
orders@antibodies.com
Produktname
Cyclin D1 (phospho Thr286) Cell Based ELISA Kit
Assay-Typ
Cell-based (qualitative)
Assay-Prinzip
This Cyclin D1 (phospho Thr286) Cell Based ELISA Kit allows for the detection of Cyclin D1 (phospho Thr286) and the effects that certain stimulation conditions have on Cyclin D1 (phospho Thr286) expression in different cell lines. Qualitative determination of Cyclin D1 (phospho Thr286) concentration is achieved by an indirect ELISA format. In essence, the Cyclin D1 (phospho Thr286) is captured by Anti-Cyclin D1 (phospho Thr286) Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this Cyclin D1 (phospho Thr286) Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) Anti-Cyclin D1 Antibody is provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.
Plattform
Microplate
Nachweismethode
Colorimetric
Probentyp
Adherent cells and suspension cells.
Detektionsbereich
> 5000 Cells
Testdauer
4 hours 30 minutes
Reaktivität
Human, Mouse, Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Lagerung
Store at + 4°C. Stable for 6 months.
Synonyms
AI327039, B cell CLL/lymphoma 1, B cell leukemia 1, B cell lymphoma 1 protein, B-cell lymphoma 1 protein, BCL 1, BCL-1, BCL-1 oncogene, BCL1, BCL1 oncogene, ccnd1, CCND1/FSTL3 fusion gene, included, CCND1/IGHG1 fusion gene, included, CCND1/IGLC1 fusion gene, included, CCND1/PTH fusion gene, included, CCND1_HUMAN, cD1, Cyl 1, D11S287E, G1/S specific cyclin D1, G1/S-specific cyclin-D1, Parathyroid adenomatosis 1, PRAD1, PRAD1 oncogene, U21B31
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

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