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Anti-Cytokeratin 20 Antikörper [ARC0288] (A306519)

Western Blot - Anti-Cytokeratin 20 Antibody [ARC0288] (A306519) - Antibodies.com Western Blot - Anti-Cytokeratin 20 Antibody [ARC0288] (A306519) - Antibodies.com Immunohistochemistry - Anti-Cytokeratin 20 Antibody [ARC0288] (A306519) - Antibodies.com
$470
Product Datasheet

Monoklonal [ARC0288] Kaninchen Antikörper gegen Cytokeratin 20 für WB und IHC.

100% Qualitätsgarantie
Preis-Match-Garantie

Versandkosten
$40
Vorlaufzeit
Lieferung in 5-8 Werktagen

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+1 (314) 370-6046
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Produktname
Anti-Cytokeratin 20 Antibody [ARC0288]
Beschreibung
Rabbit monoclonal [ARC0288] antibody to Cytokeratin 20.
Anwendungen
WB, IHC
Verdünnungen
WB: 1:500-1:2,000, IHC: 1:50-1:200
Reaktivität
Human, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 325-424 of human Cytokeratin 20 (KRT20) (P35900).
Sequenz
LANLQSLLSSLEAQLMQIRSNMERQNNEYHILLDIKTRLEQEIATYRRLLEGEDVKTTEYQLSTLEERDIKKTRKIKTVVQEVVDGKVVSSEVKEVEENI
Wirt
Rabbit
Klonalität
Monoclonal
Klon
ARC0288
Isotyp
IgG
Konjugat

Unconjugated

Reinigung
Affinity purification.
Molekulargewicht
44 kDa
Produktform
Liquid
Formulierung
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyme
CD20, CK 20, CK-20, CK20, Cytokeratin-20, Cytokeratin20, K1C20_HUMAN, K20, KA20, Keratin, Keratin 20, keratin 20, type I, keratin 21, rat, homolog of, Keratin type I cytoskeletal 20, Keratin-20, Keratin20, KRT 20, KRT 21, KRT20, KRT21, MGC35423, OTTHUMP00000164518, Protein IT, type I cytoskeletal 20
Isotyp-Kontrollantikörper
Kompatible Sekundärantikörper
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.
Western Blot - Anti-Cytokeratin 20 Antibody [ARC0288] (A306519) - Antibodies.comBild Vergrößern
Figure 1: Western Blot - Anti-Cytokeratin 20 Antibody [ARC0288] (A306519)
Western blot analysis of extracts of HT-29 cells, using Anti-Cytokeratin 20 Antibody [ARC0288] (A306519) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Western Blot - Anti-Cytokeratin 20 Antibody [ARC0288] (A306519) - Antibodies.comBild Vergrößern
Figure 2: Western Blot - Anti-Cytokeratin 20 Antibody [ARC0288] (A306519)
Western blot analysis of extracts of Rat liver, using Anti-Cytokeratin 20 Antibody [ARC0288] (A306519) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.
Immunohistochemistry - Anti-Cytokeratin 20 Antibody [ARC0288] (A306519) - Antibodies.comBild Vergrößern
Figure 3: Immunohistochemistry - Anti-Cytokeratin 20 Antibody [ARC0288] (A306519)
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Cytokeratin 20 Antibody [ARC0288] (A306519) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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