Anti-FACL4 Antikörper [ARC53209] (A306923)

Name: Anti-FACL4 Antibody [ARC53209]
Brand: Antibodies.com
SKU: A306923
Price: 470.0 USD
Availability: InStock

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$470

Monoklonal [ARC53209] Kaninchen Antikörper gegen FACL4 für WB, IHC, ICC/IF und IP.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

Garantien

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Produktdetails

Produktname

Anti-FACL4 Antibody [ARC53209]

Beschreibung

Rabbit monoclonal [ARC53209] antibody to FACL4.

Anwendungen

WB, IHC, ICC/IF, IP

Verdünnungen

WB: 1:2,000-1:4,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reaktivität

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 42-321 of human ACSL4 (NP_075266.1).

Sequenz

MAKRIKAKPTSDKPGSPYRSVTHFDSLAVIDIPGADTLDKLFDHAVSKFGKKDSLGTREILSEENEMQPNGKVFKKLILGNYKWMNYLEVNRRVNNFGSGLTALGLKPKNTIAIFCETRAEWMIAAQTCFKYNFPLVTLYATLGKEAVVHGLNESEASYLITSVELLESKLKTALLDISCVKHIIYVDNKAINKAEYPEGFEIHSMQSVEELGSNPENLGIPPSRPTPSDMAIVMYTSGSTGRPKGVMMHHSNLIAGMTGQCERIPGLGPKDTYIGYLPL

Wirt

Rabbit

Klonalität

Monoclonal

Klon

ARC53209

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity purification.

Molekulargewicht

79 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

ACS 4, ACS4, ACSL 4, Acsl4, ACSL4_HUMAN, acyl CoA synthetase 4, Acyl CoA synthetase long chain family member 4, FACL 4, Fatty acid Coenzyme A ligase, fatty acid Coenzyme A ligase long-chain 4, LACS 4, LACS4, Lignoceroyl CoA synthase, Long chain 4, long chain acyl CoA synthetase 4, long chain fatty acid CoA ligase 4, long chain fatty acid Coenzyme A ligase 4, Long-chain acyl-CoA synthetase 4, Long-chain-fatty-acid--CoA ligase 4, MRX63, MRX68

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (10)

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Figure 1: Western Blot - Anti-FACL4 Antibody [ARC53209] (A306923)

Western blot analysis of extracts of various lysates, using Anti-FACL4 Antibody [ARC53209] (A306923) at 1:4,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 0. 3s.

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Figure 2: Western Blot - Anti-FACL4 Antibody [ARC53209] (A306923)

Western blot analysis of extracts of Rat liver, using Anti-FACL4 Antibody [ARC53209] (A306923) at 1:24000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 3: Western Blot - Anti-FACL4 Antibody [ARC53209] (A306923)

Western blot analysis of extracts of Mouse liver, using Anti-FACL4 Antibody [ARC53209] (A306923) at 1:24000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 4: Immunohistochemistry - Anti-FACL4 Antibody [ARC53209] (A306923)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-FACL4 Antibody [ARC53209] (A306923) at a dilution of 1:200(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 5: Immunohistochemistry - Anti-FACL4 Antibody [ARC53209] (A306923)

Immunohistochemistry analysis of paraffin-embedded human lung using Anti-FACL4 Antibody [ARC53209] (A306923) at a dilution of 1:200(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 6: Immunohistochemistry - Anti-FACL4 Antibody [ARC53209] (A306923)

Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-FACL4 Antibody [ARC53209] (A306923) at a dilution of 1:200(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 7: Immunohistochemistry - Anti-FACL4 Antibody [ARC53209] (A306923)

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using Anti-FACL4 Antibody [ARC53209] (A306923) at a dilution of 1:200(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 8: Immunohistochemistry - Anti-FACL4 Antibody [ARC53209] (A306923)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-FACL4 Antibody [ARC53209] (A306923) at a dilution of 1:200(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 9: Immunofluorescence - Anti-FACL4 Antibody [ARC53209] (A306923)

Confocal imaging of HepG2 cells using Anti-FACL4 Antibody [ARC53209] (A306923), at a dilution of 1:1000, (red). DAPI was used for nuclear staining (Blue). Objective: 60x.

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Figure 10: Western Blot - Anti-FACL4 Antibody [ARC53209] (A306923)

Immunoprecipitation analysis of 300µg extracts of HepG2 cells using 3µg of Anti-FACL4 Antibody [ARC53209] (A306923). This Western blot was performed on the immunoprecipitate using Anti-FACL4 Antibody [ARC53209] (A306923) at a dilution of 1:1000.

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